tailieunhanh - Báo cáo y học: "Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry."

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry. | Chinese Medicine BioMed Central Research Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry Fengguo Xu1 2 Ying Liu3 Zunjian Zhang 1 2 Cheng Yang1 2 and Yuan Tian1 2 Address 1Key Laboratory of Drug Quality Control and Pharmacovigilance Ministry of Education China Pharmaceutical University Nanjing Jiangsu 210009 PR China 2Center for Instrumental Analysis China Pharmaceutical University Nanjing Jiangsu 210009 PR China and 3Department of Pharmacy Nanjing Municipal Hospital of Traditional Chinese Medicine Nanjing Jiangsu 210001 PR China Email Fengguo Xu - fengguoxu@ Ying Liu - Zunjian Zhang - zunjianzhangcpu@ Cheng Yang - ycmake@ Yuan Tian - tiancpu@ Corresponding author Open Access Published 24 July 2009 Received 20 December 2008 Chinese Medicine 2009 4 15 doi 1749-8546-4-15 Accepted 24 July 2009 This article is available from http content 4 1 15 2009 Xu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Da Chengqi Tang DCT is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived from Fructus Aurantii Immaturus. The present study developed an LC-MS MS method to characterize two pairs of flavanone 7-glycoside isomers . hesperidin versus neohesperidin and naringin versus isonaringin. Methods After solid phase purification components in sample were separated on a Agilent zorbax SB-C18 5 pm 250 mm X mm analytical column. ESI-MS and quasi-MSn were performed in negative ion mode to obtain structural data of .