tailieunhanh - Báo cáo y học: "In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures. | Chinese Medicine BioMed Central Research In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma Royle Johnst Ruanzicao cell suspension cultures Wenbi Xiong Gang Luo Liming Zhou Yun Zeng and Wenji Yang Address Department of Pharmacology West China Center for Medical Sciences Sichuan University Chengdu Sichuan 610041 PR China Email Wenbi Xiong - xiongwbcd@ Gang Luo - luogang2003@ Liming Zhou - zhou108@ Yun Zeng - zeng_yun@ Wenji Yang - wenji@ Corresponding author Open Access Published II July 2009 Received 25 September 2008 Chinese Medicine 2009 4 14 doi 1749-8546-4-14 Accepted 11 July 2009 This article is available from http content 4 1 14 2009 Xiong et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb Arnebia euchroma Royle Johnst. Methods The 3- 4 5-dimethylthiazol- 2-yl -2 5-diphenyltetrazolium bromide MTT assay was used to determine the in vitro antitumor effects of acetylshikonin on human lung adenocarcinoma cell line A549 human hepatocellular carcinoma cell line Bel-7402 human breast adenocarcinoma cell line MCF-7 and mouse Lewis lung carcinoma LLC cell line. C57BL 6 mice with LLC model were used to study the in vivo antitumor effects of acetylshikonin. The expression of bax bcl-2 and caspase-3 proteins in LLC tissue was determined with immunohistochemical staining. Results In A549 Bel-7402 MCF-7 and LLC cell lines acetylshikonin inhibited cell growth in a