tailieunhanh - Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry. | Liang et al. Chinese Medicine 2010 5 29 http content 5 1 29 CHINESE MEDICINE RESEARCH Open Access Comparison of raw and processed Radix Polygoni Multiflori Heshouwu by high performance liquid chromatography and mass spectrometry Zhitao Liang Hubiao Chen Zhiling Yu Zhongzhen Zhao Abstract Background Radix Polygon Multiflori is the dried root tuber of Polygonum multiflorum Thunb. Fam. Polygonaceae . According to Chinese medicine theory raw R-RPM and processed P-RPM Radix Polygoni Multiflori possess different properties. The present study investigates the differences in chemistry between raw and processed Radix Polygoni Multiflori. Methods Five pairs of R-RPM and P-RPM as well as 15 commercial decoction pieces were analyzed with high performance liquid chromatography HPLC and mass spectrometry MS . Results Two anthraquinones namely emodin-8-O- 6 -O-malonyl -glucoside and physcion-8-O- 6 -O-malonyl -glucoside disappeared or decreased significantly and 2 3 5 4 -tetrahydroxystilbene-2-O-j8-D-glucopyranoside emodin-8-O-b-D-glucopyranoside and physcion-8-O-b-D-glucopyranoside decreased after the R-RPM samples being processed. On the other hand the contents of emodin and physcion generally increased after processing. Conclusion The present study indicates that processing Radix Polygoni Multiflori may change the contents and types of chemicals in it. These changes are probably responsible for the various pharmacological effects of R-RPM and P-RPM as well as hepatotoxicity. Background Proper pharmaceutical processing may reduce toxicity or side effects potentiate the beneficial effects change the pharmacological properties preserve active constituents facilitate administration improve flavor or correct unpleasant taste and increase purity of Chinese materia medica 1-4 . In China the processing methods for Radix Polygoni Multiflori have been practiced since the Tang dynasty 5 and are documented in the Chinese pharmacopoeia 6 . Radix Polygoni Multiflori .