tailieunhanh - Báo cáo y học: "enetic variability and population structure of endangered Panax ginseng in the Russian Primorye"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Genetic variability and population structure of endangered Panax ginseng in the Russian Primorye. | Zhuravlev et al. Chinese Medicine 2010 5 21 http content 5 1 21 CHINESE MEDICINE RESEARCH Open Access Genetic variability and population structure of endangered Panax ginseng in the Russian Primorye Yuri N Zhuravlev 1 Galina D Reunova1 Irina L Kats1 Tamara I Muzarok1 and Alexander A Bondar2 Abstract Background The natural habitat of wild Pginseng is currently found only in the Russian Primorye and the populations are extremely exhausted and require restoration. Analysis of the genetic diversity and population structure of an endangered species is a prerequisite for conservation. The present study aims to investigate the patterns and levels of genetic polymorphism and population structures of wild P ginseng with the AFLP method to 1 estimate the level of genetic diversity in the Pginseng populations in the Russian Primorsky Krai 2 calculate the distribution of variability within a population and among populations and 3 examine the genetic relationship between the populations. Methods Genetic variability and population structure of ten Pginseng populations were investigated with Amplified Fragment Length Polymorphism AFLP markers. The genetic relationships among Pginseng plants and populations were delineated. Results The mean genetic variability within populations was high. The mean level of polymorphisms was at the population level and at the species level. The Shannon s index ranged between and with an average of at the population level and at the species level. The analysis of molecular variances AMOVA showed a significant population structure in P ginseng. The partition of genetic diversity with AMOVA suggested that the majority of the genetic variation was within populations of P ginseng. The inter-population variability was approximately 36 of the total variability. The genetic relationships among Pginseng plants and populations were reconstructed by Minimum Spanning tree MS-tree on the basis of .