tailieunhanh - Báo cáo y học: " Research Evaluation of the collaborative network of highly correlating skin protein"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Research Evaluation of the collaborative network of highly correlating skin proteins. | Klinge et al. Theoretical Biology and Medical Modelling 2010 7 16 http content 7 1 16 THEORETICAL BIOLOGY AND MEDICAL MODELLING RESEARCH Open Access Evaluation ofthe collaborative network of highly correlating skin proteins and its change following treatment with glucocorticoids Uwe Klinge 1 2 Nicolette Farman3 and Anette Fiebeler4 Correspondence Uklinge@ 1 Surgical Department of the University Hospital of the RWTH Aachen Germany Full list of author information is available at the end of the article Abstract Background Glucocorticoids GC represent the core treatment modality for many inflammatory diseases. Its mode of action is difficult to grasp not least because it includes direct modulation of many components of the extracellular matrix as well as complex antiinflammatory effects. Protein expression profile of skin proteins is being changed with topical application of GC however the knowledge about singular markers in this regard is only patchy and collaboration is ill defined. Material Methods Scar formation was observed under different doses of GC which were locally applied on the back skin of mice 1 to 3 weeks . After euthanasia we analyzed protein expression of collagen I and III picrosirius in scar tissue together with 16 additional protein markers which are involved in wound healing with immunhistochemistry. For assessing GC s effect on co-expression we compared our results with a model of random figures to estimate how many significant correlations should be expected by chance. Results GC altered collagen and protein expression with distinct results in different areas of investigation. Most often we observed a reduced expression after application of low dose GC. In the scar infiltrate a multivariate analysis confirmed the significant impact of both GC concentrations. Calculation of Spearman s correlation coefficient similarly resulted in a significant impact of GC and furthermore offered the possibility to grasp the entire .