tailieunhanh - Advances in Solid State Part 15

Tham khảo tài liệu 'advances in solid state part 15', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Miniature Dual Axes Confocal Microscope for Real Time In Vivo Imaging 411 direction over a depth of 500 pm. Over the right half of the image vertically oriented crypts with individual mucin-secreting goblet cells associated with intestinal metaplasia can be appreciated as brightly stained vacuoles. This diseased condition is associated with greater than 100 fold relative risk of developing cancer in the esophagus. In Fig. 13b the specimen was collected from a flat colonic adenoma and the image reveals vertically oriented dysplastic crypts with individual goblet cells. Volume rendering can also be performed with the dual axes confocal microscope to illustrate three-dimensional 3D imaging capabilities. These views are important for tracking cell movements observing protein-protein interactions and monitoring angiogenic development. A xenograft mouse model of glioblastoma multiforme has been developed by subcutaneously implanting 107 human U87MG glioblastoma cells in the flank of a nude mouse. Horizontal cross-sectional fluorescence images were collected with a tabletop instrument when the tumors reached 1 cm in size. The mice were anesthetized for the in vivo imaging session and indocyanine green ICG at a concentration of mg ml was injected intravenously to produce contrast. A skin flap overlying the tumor was exposed and horizontal cross-sectional images were collected with a FOV of 400 x 500 pm2. A fluorescence image collected at 50 pm below the tissue surface shown in Fig. 14a reveals that the glioblastoma has developed a dense complex network of tortuous vasculature. A total of 400 horizontal cross-sectional images acquired at 1 pm increments were used to Fig. 14. Dual axes confocal fluorescence images in small animal models. a A horizontal cross-sectional fluorescence image of a human U87MG xenograft glioblastoma tumor implanted subcutaneously in the flank of a nude mouse was collected in vivo at 50 pm depth using . indocyanine green ICG . b A 3D .