tailieunhanh - Báo cáo y học: "Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS). | Zhao and Leung Chinese Medicine 2010 5 4 http content 5 1 4 CHINESE MEDICINE RESEARCH Open Access Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography HPLC coupled with diode array detector and mass spectrometry DAD-MS Sandy Shuo Zhao Kelvin Sze-Yin Leung Abstract Background Antrodia camphorata AC is an important fungus native to Taiwanese forested regions. Scientific studies have demonstrated that extracts of AC possess a variety of pharmacological functions. This study aims to identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC and to assess the quality of two commercial mycelial AC products. Methods High-performance liquid chromatography coupled with diode array detector and mass spectrometry was employed to identify the major components in mycelial AC. The chemical separation was carried out using a gradient program on a reverse phase Alltima C18 AQ analytical column 250 X mm 5 gm with the mobile phase consisting of deionized water and methanol. Results Ten nucleosides and nucleobases two maleimide derivatives and a sterol were identified as the major constituents in mycelial AC. These groups of chemical compounds constitute the first chromatographic fingerprint as an index for quality assessment of this medicinal fungus. Conclusions This study provides the first chromatographic fingerprint to assess the quality of mycelial AC. Background Antrodia camphorata M. Zang . Su Sheng H. Wu Ryvarden . Chang Polyporaceae is a parasitic fungus on decayed wood or the inner wall of the heartwood of Cinnamomum kanehirai hay a tree endemic to Taiwan. Before Antrodia camphorata AC was first officially classified as a species in 1990 its medicinal value had been greatly appreciated for many decades. This highly valuable fungus is widely recommended by the traditional Chinese medicine practitioners for food intoxication vomiting and poisoning 1 . In addition it was shown .