tailieunhanh - Verlinden et al. AMB Express 2011, 1:11 http://www.amb-express.com/content/1/1/11 ORIGINAL Open

Verlinden et al. AMB Express 2011, 1:11 ORIGINAL Open Access Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator Rob AJ Verlinden1, David J Hill1, Melvin A Kenward1, Craig D Williams1, Zofia Piotrowska-Seget2 and Iza K Radecka1* Abstract Polyhydroxyalkanoates (PHAs) are biopolymers, which can replace petrochemical plastics in many applications. However, these bioplastics are currently far more expensive than petrochemical plastics. Many researchers are investigating the use of inexpensive substrates derived from waste streams. Waste frying oil is abundant and can be used in PHA production without filtration. Cupriavidus necator (formerly known as Ralstonia eutropha) is a versatile organism for the production of. | Verlinden et al. AMB Express 2011 1 11 http content 1 1 11 o AMB Express a SpringerOpen Journal ORIGINAL Open Access Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator Rob AJ Verlinden1 David J Hill1 Melvin A Kenward1 Craig D Williams1 Zofia Piotrowska-Seget2 and Iza K Radecka1 Abstract Polyhydroxyalkanoates PHAs are biopolymers which can replace petrochemical plastics in many applications. However these bioplastics are currently far more expensive than petrochemical plastics. Many researchers are investigating the use of inexpensive substrates derived from waste streams. Waste frying oil is abundant and can be used in PHA production without filtration. Cupriavidus necator formerly known as Ralstonia eutropha is a versatile organism for the production of PHAs. Small-scale batch fermentation studies have been set up using different concentrations of pure vegetable oil heated vegetable oil and waste frying oil. These oils are all rapeseed oils. It has been shown that Cupriavidus necator produced the homopolymer polyhydroxybutyrate PHB from the rapeseed oils. The achieved PHB concentration from waste frying oil was g l which is similar to a concentration that can be obtained from glucose. The PHB harvest from pure oil and heated oil was g l and g l respectively. A feed of waste frying oil could thus achieve more biopolymer than pure vegetable oil. While the use of a waste product is beneficial from a life-cycle perspective PHB is not the only product that can be made from waste oil. The collection of waste frying oil is becoming more widespread making waste oil a good alternative to purified oil or glucose for PHB production. Keywords polyhydroxyalkanoates polyhydroxybutyrate bacterial fermentation biopolymer waste frying oil rapeseed oil Introduction Polyhydroxyalkanoates PHAs are natural renewable and biocompatible biopolymers produced intracellular in bacteria. They can be made into plastic materials .