tailieunhanh - Báo cáo y học: "Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke. | Zhang et al. Chinese Medicine 2010 5 35 http content 5 1 35 CHINESE MEDICINE RESEARCH Open Access Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke 13 4 1 1 1 Lihong Zhang Wai Ping Lam Lanhai Lu Chunmei Wang Yeuk Wa Wong Lok Hang Lam Hong Chai Tang 1 2 1 41 1 Maria Sen Mun Wai Mingwei Wang Wing Hang Kwong Sai Ming Ngai Ying Tat Mak David Tai Wai Yew Abstract Background Stroke caused by brain ischemia is the third leading cause of adult disability. Active prevention and early treatment of stroke targeting the causes and risk factors may decrease its incidence mortality and subsequent disability. Pien Tze Huang PZH a Chinese medicine formula was found to have anti-edema antiinflammatory and anti-thrombotic effects that can prevent brain damage. This study aims to investigate the potential mechanisms of the preventive effects of Pien Tze Huang on brain damage caused by chronic ischemia and hypertensive stroke in rats. Methods The effects of Pien Tze Huang on brain protein expression in spontaneously hypertensive rat SHR and stroke prone SHR SHRsp were studied with 2-D gel electrophoresis and mass spectrometric analysis with a matrix-assisted laser desorption ionization time-of-flight MALDI-TOF TOF tandem mass spectrometer and on brain cell death with enzyme link immunosorbent assay ELISA and immunostaining. Results Pien Tze Huang decreased cell death in hippocampus and cerebellum caused by chronic ischemia and hypertensive stroke. Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia. Brain protein expression results suggested that Pien Tze Huang downregulated QCR2 in the electron transfer chain of mitochondria preventing reactive oxygen species ROS damage and possibly subsequent cell death caspase 3 assay as caused by chronic ischemia or hypertensive stroke to hippocampus and cerebellum. Conclusion Pien Tze Huang .