tailieunhanh - Báo cáo khoa học: New insights into evolution of crustacean hyperglycaemic hormone in decapods – first characterization in Anomura

The neuropeptides of the crustacean hyperglycaemic hormone (CHH) family are encoded by a multigene family and are involved in a wide spec-trum of essential functions. In order to characterize CHH family peptides in one of the last groups of decapods not yet investigated, CHH was stud-ied in two anomurans: the hermit crab Pagurus bernhardusand the squat lobster Galathea strigosa. | ỊFEBS Journal New insights into evolution of crustacean hyperglycaemic hormone in decapods - first characterization in Anomura Nicolas Montagne Daniel Soyez Dominique Gallois Celine Ollivaux and Jean-Yves Toullec Université Pierre et Marie Curie - Paris 6 FRE 2852 CNRS - Proteines Biochimie Structurale et Fonctionnelle Equipe Biogenese des Peptides Isomeres Paris France Keywords Anomura CHH CPRP molecular evolution neuropeptide Correspondence N. Montagne Equipe Biogenese des Peptides Isomeres - FRE 2852 CNRS Universite Pierre et Marie Curie 7 Quai Saint-Bernard 75252 Paris Cedex 05 France Fax 33 1 44 27 23 61 Tel 33 1 44 27 22 53 E-mail Database Sequences for P. bernhardus CHH and G. strigosa CHH have been submitted to the GenBank database under the accession numbers DQ450960 and EF492145 respectively Received 11 October 2007 revised 4 December 2007 accepted 17 December 2007 doi The neuropeptides of the crustacean hyperglycaemic hormone CHH family are encoded by a multigene family and are involved in a wide spectrum of essential functions. In order to characterize CHH family peptides in one of the last groups of decapods not yet investigated CHH was studied in two anomurans the hermit crab Pagurus bernhardus and the squat lobster Galathea strigosa. Using RT-PCR and 3 and 5 RACE methods a preproCHH cDNA was cloned from the major neuroendocrine organs X-organs of these two species. Hormone precursors deduced from these cDNAs in P. bernhardus and G. strigosa are composed of signal peptides of 29 and 31 amino acids respectively and CHH precursor-related peptides CPRPs of 50 and 40 amino acids respectively followed by a mature hormone of 72 amino acids. The presence of these predicted CHHs and their related CPRPs was confirmed by performing MALDI-TOF mass spectrometry on sinus glands the main neurohaemal organs of decapods. These analyses also suggest the presence in sinus glands of both species of a .