tailieunhanh - Báo cáo khoa học: Abundance of intrinsic disorder in SV-IV, a multifunctional androgen-dependent protein secreted from rat seminal vesicle

The potent immunomodulatory, anti-inflammatory and procoagulant properties of protein no. 4 secreted from the rat seminal vesicle epithelium (SV-IV) have previously been found to be modulated by a supramolecular monomer–trimer equilibrium. | ỊFEBS Journal Abundance of intrinsic disorder in SV-IV a multifunctional androgen-dependent protein secreted from rat seminal vesicle Silvia Vilasi and Raffaele Ragone Dipartimento di Biochimica e Biofisica Naples Italy Keywords bioinformatics disorder prediction intrinsically disordered proteins seminal vesicle protein no. 4 structure-function relationship Correspondence R. Ragone Dipartimento di Biochimica e Biofisica Seconda Universita di Napoli via S. Maria di Costantinopoli 16 80138 Naples Italy Fax 39 081 294136 Tel 39 081 294042 E-mail raffrag@ Received 30 October 2007 revised 5 December 2007 accepted 13 December 2007 doi The potent immunomodulatory anti-inflammatory and procoagulant properties of protein no. 4 secreted from the rat seminal vesicle epithelium SV-IV have previously been found to be modulated by a supramolecular monomer-trimer equilibrium. More structural details that integrate experimental data into a predictive framework have recently been reported. Unfortunately homology modelling and fold-recognition strategies were not successful in creating a theoretical model of the structural organization of SV-IV. It was inferred that the global structure of SV-IV is not similar to that of any protein of known three-dimensional structure. Reversing the classical approach to the sequence-structure-function paradigm in this paper we report novel information obtained by comparing the physicochemical parameters of SV-IV with two datasets composed of intrinsically unfolded and ideally globular proteins. In addition we analyse the SV-IV sequence by several publicly available disorder-oriented predictors. Overall disorder predictions and a re-examination of existing experimental data strongly suggest that SV-IV needs large plasticity to efficiently interact with the different targets that characterize its multifaceted biological function and should therefore be better classified as an .