tailieunhanh - Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress

The rapid increase in the number of putative cDNA sequences encoding crustacean hyperglycemic hormone (CHH) family in various tissues [either from the eyestalk (ES) or elsewhere] underscores a need to identify the corresponding neuropeptides in relevant tissues. | ỊFEBS Journal Functional studies of crustacean hyperglycemic hormones CHHs of the blue crab Callinectes sapidus - the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress J. Sook Chung and N. Zmora Center of Marine Biotechnology University of Maryland Biotechnology Institute Baltimore USA Keywords hypoxia pericardial organ and sinus gland crustacean hyperglycemic hormones temperature stress Correspondence J. S. Chung Center of Marine Biotechnology University of Maryland Biotechnology Institute 701 East Pratt Street Columbus Center Suite 236 Baltimore MD 21202 USA Fax 1 410 234 8896 Tel 1 410 234 8841 E-mail chung@ Received 9 October 2007 revised 5 December 2007 accepted 11 December 2007 doi The rapid increase in the number of putative cDNA sequences encoding crustacean hyperglycemic hormone CHH family in various tissues either from the eyestalk ES or elsewhere underscores a need to identify the corresponding neuropeptides in relevant tissues. Moreover the presence of provided structural CHH implies the level of the complexity of physiological regulation in crustaceans. Much less is known of the functions of nonES CHH than of those of its counterpart present in ESs. In the blue crab Callinectes sapidus we know little of CHH involvement in response to the stressful conditions that naturally occur in Chesapeake Bay. We have identified two isoforms of CHH neuropeptide in the sinus gland of the ES and isolated a full-length cDNA encoding CHH from the pericardial organ PO . The functions of ES-CHH and PO-CHH in this species were studied with regard to expression and release in response to stressful episodes hypoxia emersion and temperatures. Animals exposed to hypoxic conditions responded with concomitant release of both CHHs. In contrast the mRNA transcripts encoding two CHHs were differentially regulated PO-CHH increased whereas ES-CHH decreased. This result suggests a .