tailieunhanh - Báo cáo y học: "Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L. | Sohn et al. Clinical and Molecular Allergy 2011 9 4 http content 9 1 4 RESEARCH CMA CLINICAL AND MOLECULAR ALLERGY Open Access Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L 112 1 3 4 5 Eun-Hwa Sohn Seon-A Jang Haemi Joo Sulkyoung Park Se-Chan Kang Chul-Hoon Lee Sun-Young Kim Abstract Background Atopic dermatitis is a chronic allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells including eosinophils mast cells and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa ALBE had previously unreported antiallergic or anti-inflammatory effects. Methods This study examined the effect of ALBE on the release of p-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4 IL-5 mitogen-activated protein kinases MAPKs and nuclear factor NF -kB using RT-PCR Western blotting and ELISA in mouse splenocytes after ALBE treatment. Results We observed significant inhibition of p-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally ALBE 100 gg mL suppressed not only the transcriptional activation of NF-kB but also the phosphorylation of MAPKs in ConA-treated primary splenocytes. Conclusions These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-kB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases including atopic dermatitis. Background Atopic dermatitis is a chronic allergic inflammatory skin disorder characterized by pruritic chronic eczema elevated serum IgE levels and massive cellular infiltrates including eosinophils mast cells and lymphocytes 1 2 . Because mast cells .