tailieunhanh - Báo cáo y học: "Patients with allergic rhinitis and allergic asthma share the same pattern of eosinophil and neutrophil degranulation after allergen challenge"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Patients with allergic rhinitis and allergic asthma share the same pattern of eosinophil and neutrophil degranulation after allergen challenge. | Kămpe et al. Clinical and Molecular Allergy 2011 9 3 http content 9 1 3 RESEARCH CMA CLINICAL AND MOLECULAR ALLERGY Open Access Patients with allergic rhinitis and allergic asthma share the same pattern of eosinophil and neutrophil degranulation after allergen challenge Mary Kampe 1 Ingrid Stolt Maria Lampinen Christer Janson 1 Gunnemar Stalenheim 1 Marie Carlson Abstract Background Patients with allergic rhinitis and allergic asthma demonstrate comparable local and systemic eosinophil inflammation and yet they present with different clinical pictures. Less is even known about the contribution of neutrophil inflammation in allergic diseases. The aim of the study was to examine the propensity and selectivity of granule release from primed systemic eosinophils and neutrophils in allergic rhinitis and allergic asthma after seasonal and experimental allergen exposure. We hypothesize that the dissimilar clinical manifestations are due to diverse eosinophil and neutrophil degranulation. Methods Nine birch pollen allergic patients with rhinitis eight with asthma and four controls were studied during pollen season and after nasal and bronchial allergen challenge. Eosinophils and neutrophils were incubated in vitro with assay buffer and opsonized Sephadex particles for spontaneous and C3b-induced granule protein release. The released amount of eosinophil cationic protein ECP eosinophil peroxidase EPO and myeloperoxidase MPO was measured by specific radioimmunoassay. Results C3b-induced degranulation resulted in increased release of ECP and MPO from primed blood eosinophils and neutrophils in both allergic rhinitis and allergic asthma during pollen season and after both nasal and bronchial challenge p-values to . After bronchial challenge the ECP release was significantly higher in the rhinitic group compared to the asthmatic group vs. p . The propensity for EPO release was weak in all .