tailieunhanh - Báo cáo khoa học: The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation

Protein arginine methyltransferase (PRMT)1 is the predominant type I methyltransferase in mammals. In the present study, we used zebrafish (Danio rerio) as the model system to elucidate PRMT1 expression and function during embryogenesis. | IFEBS Journal The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation Yun-Jung Tsai1 Huichin Pan1 2 Chuan-Mao Hung1 Po-Tsun Hou1 Yi-Chen Li1 Yu-Jen Lee3 Yi-Ting Shen1 4 Trang-Tiau Wu4 5 and Chuan Li1 2 1 Department of BiomedicalSciences Chung Shan MedicalUniversity Taichung Taiwan 2 Department of MedicalResearch Chung Shan MedicalUniversity Hospital Taichung Taiwan 3 Institute of Biochemistry and Biotechnology Chung Shan MedicalUniversity Taichung Taiwan 4 Department of Pediatric Surgery Chung Shan MedicalUniversity Hospital Taichung Taiwan 5 Schoolof Medicine Chung Shan MedicalUniversity Taichung Taiwan Keywords convergence and extension gastrulation PRMT1 protein arginine methylation zebrafish Correspondence C. Li Department of Biomedical Sciences Chung Shan MedicalUniversity Taichung Taiwan Fax 886 4 23248187 Tel 886 4 24730022 11807 E-mail cli@ Received 27 August 2010 revised 19 December 2010 accepted 5 January 2011 doi Protein arginine methyltransferase PRMT 1 is the predominant type I methyltransferase in mammals. In the present study we used zebrafish Danio rerio as the model system to elucidate PRMT1 expression and function during embryogenesis. Zebrafish prmtl transcripts were detected from the zygote period to the early larva stage. Knockdown of prmtl by antisense morpholino oligo AMO resulted in delayed growth shortened body-length curled tails and cardiac edema. PRMT1 protein level type I protein arginine methyltransferase activity specific asymmetric protein arginine methylation and histone H4 R3 methylation all decreased in the AMO-injected morphants. The morphants showed defective convergence and extension and the abnormalities were more severe at the posterior than the anterior parts. Cell migration defects suggested by the phenotypes were not only observed in the morphant embryos but also in a cellular prmtl small-interfering RNA .