tailieunhanh - Báo cáo y học: "Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets. | Chirumbolo et al. Clinical and Molecular Allergy 2010 8 13 http content 8 1 13 RESEARCH CMA CLINICAL AND MOLECULAR ALLERGY Open Access Bimodal action of the flavonoid quercetin on basophil function an investigation of the putative biochemical targets 1 1 A . z- r . 2 3 3 1 Salvatore Chirumbolo Marta Marzotto Anita Conforti Antonio Vella Riccardo Ortolani Paolo Bellavite Abstract Background Flavonoids a large group of polyphenolic metabolites derived from plants have received a great deal of attention over the last several decades for their properties in inflammation and allergy. Quercetin the most abundant of plant flavonoids exerts a modulatory action at nanomolar concentrations on human basophils. As this mechanism needs to be elucidated in this study we focused the possible signal transduction pathways which may be affected by this compound. Methods K2-EDTA derived leukocyte buffy coats enriched in basophil granulocytes were treated with different concentrations of quercetin and triggered with anti-IgE fMLP the calcium ionophore A23187 and the phorbol ester PMA in different experimental conditions. Basophils were captured in a flow cytometry analysis as CD123bright HLADRnon expressing cells and fluorescence values of the activation markers CD63-FITC or CD203c-PE were used to produce dose response curves. The same population was assayed for histamine release. Results Quercetin inhibited the expression of CD63 and CD203c and the histamine release in basophils activated with anti-IgE or with the ionophore the IC50 in the anti-IgE model was higher than in the ionophore model and the effects were more pronounced for CD63 than for CD203c. Nanomolar concentrations of quercetin were able to prime both markers expression and histamine release in the fMLP activation model while no effect of quercetin was observed when basophils were activated with PMA. The specific phosphoinositide-3 kinase PI3K inhibitor wortmannin exhibited the same .