tailieunhanh - Báo cáo y học: "Differential effects of Radix Paeoniae Rubra (Chishao) on cytokine and"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Differential effects of Radix Paeoniae Rubra (Chishao) on cytokine and . | Wang et al. Chinese Medicine 2011 6 14 http content 6 1 14 CHINESE MEDICINE RESEARCH Open Access Differential effects of Radix Paeoniae Rubra Chishao on cytokine and chemokine expression inducible by mycobacteria Liangjie Wang 1 Cindy Lai Hung Yang1 Terry Cho Tsun Or2 Gang Chen4 Jian Zhou4 James Chun Bong Li1 2 and Allan Sik Yin Lau1 2 3 Abstract Background Upon initial infection with mycobacteria macrophages secrete multiple cytokines and chemokines including interleukin-6 IL-6 IL-8 and tumor necrosis factor-a TNF-a to mediate host immune responses against the pathogen. Mycobacteria also induce the production of IL-10 via PKR activation in primary human monocytes and macrophages. As an anti-inflammatory cytokine over-expression of IL-10 may contribute to mycobacterial evasion of the host immunity. Radix Paeoniae Rubra RPR Chishao a Chinese medicinal herb with potentials of antiinflammatory hepatoprotective and neuroprotective effects is used to treat tuberculosis. This study investigates the immunoregulatory effects of RPR on primary human blood macrophages PBMac during mycobacterial infection. Methods The interaction of Bacillus Calmette-Guerin BCG with PBMac was used as an experimental model. A series of procedures involving solvent extraction and fractionation were used to isolate bioactive constituents in RPR. RPR-EA-S1 a fraction with potent immunoregulatory effects was obtained with a bioactivity guided fractionation scheme. PBMac were treated with crude RPR extracts or RPR-EA-S1 before BCG stimulation. The expression levels of IL-6 IL-8 IL-10 and TNF-a were measured by qPCR and ELISA. Western blotting was used to determine the effects of RPR-EA-S1 on signaling kinases and transcriptional factors in the BCG-activated PBMac. Results In BCG-stimulated macrophages crude RPR extracts and fraction RPR-EA-S1 specifically inhibited IL-10 production while enhanced IL-8 expression at both mRNA and protein levels without affecting the expressions of