tailieunhanh - Báo cáo y học: "Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes. | Tsai et al. Chinese Medicine 2011 6 12 http content 6 1 12 CHINESE MEDICINE RESEARCH Open Access Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes Wei-Jern Tsai1 Chu-Ting Chang2 Guei-Jane Wang1 Tzong-Huei Lee3 Shwu-Fen Chang4 Shao-Chun Lu5 and Yuh-Chi Kuo2 Abstract Background Arctium lappa Niubang a Chinese herbal medicine is used to treat tissue inflammation. This study investigates the effects of arctigenin AC isolated from A. lappa on anti-CD3 CD28 Ab-stimulated cell proliferation and cytokine gene expression in primary human T lymphocytes. Methods Cell proliferation was determined with enzyme immunoassays and the tritiated thymidine uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction. Results AC inhibited primary human T lymphocytes proliferation activated by anti-CD3 CD28 Ab. Cell viability test indicated that the inhibitory effects of AC on primary human T lymphocyte proliferation were not due to direct cytotoxicity. AC suppressed interleukin-2 IL-2 and interferon-y IFN-y production in a concentration-dependent manner. Furthermore AC decreased the IL-2 and IFN-y gene expression in primary human T lymphocytes induced by anti-CD3 CD28 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression. Conclusion AC inhibited T lymphocyte proliferation and decreased the gene expression of IL-2 IFN-y and NF-AT. Background The central event in the generation of immune responses is the activation and clonal expansion of T cells. Interaction of T cells with antigens initiates a cascade of biochemical events and gene expression that induces the resting T cells to activate and proliferate 1 . Activation of nuclear factor of activated T cells NF-AT and a series of genes such as interleukin-2 IL-2 and interferon-y IFN-y are pivotal in the growth of T lymphocytes induced by antigens 2 3 . Thus