tailieunhanh - Báo cáo y học: "Association of IL-4RA single nucleotide polymorphisms, HLA-DR and HLA-DQ in children with Alternaria-sensitive moderate-severe asthm"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Association of IL-4RA single nucleotide polymorphisms, HLA-DR and HLA-DQ in children with Alternaria-sensitive moderate-severe asthma. | Knutsen et al. Clinical and Molecular Allergy 2010 8 5 http content 8 1 5 RESEARCH CMA CLINICAL AND MOLECULAR ALLERGY Open Access Association of IL-4RA single nucleotide polymorphisms HLA-DR and HLA-DQ in children with Alternaria-sensitive moderate-severe asthma A I D IX I I r-f-t 1 3 _I LTI ft I Í I I5 D I rii 11 1 1 3 I I I 1I- c I z t wx2 D I L fZ. r pp2 I wx p A -vplpz-v rp 1 Alan P Knutsen Hari M Vijay Barbara Kariuki Luis A Santiago Ralph Graff Jonathan D VVOTTOIQ Maulik R Shah1 4 Abstract Background Asthma afflicts 6 to 8 of the United States population and severe asthma represents approximately 10 of asthmatic patients. Several epidemiologic studies in the United States and Europe have linked Alternaria sensitivity to both persistence and severity of asthma. In order to begin to understand genetic risk factors underlying Alternaria sensitivity and asthma in these studies we examined T cell responses to Alternaria antigens HLA Class II restriction and HLA-DQ protection in children with severe asthma. Methods Sixty children with Alternaria-sensitive moderate-severe asthma were compared to 49 children with Alternaria-sensitive mild asthma. We examined HLA-DR and HLA-DQ frequencies in Alternaria-sensitive asthmatic by HLA typing. To determine ratios of Th1 Th2 Alternaria-specific T-cells cultures were stimulated in media alone Alternaria alternata extract and Alt a1. Sensitivity to IL-4 stimulation was measured by up-regulation of CD23 on B cells. Results Children with Alternaria-sensitive moderate-severe asthma trended to have increased sensitivities to Cladosporium 46 versus 35 to Aspergillus 43 versus 28 and significantly increased sensitivities to trees 78 versus 57 and to weeds 68 versus 48 . The IL-4RA ile75val polymorphism was significantly increased in Alternaria-sensitive moderate-severe asthmatics 83 allele frequency compared to Alternaria-sensitive mild asthmatics 57 allele frequency . This was associated