tailieunhanh - Báo cáo y học: " Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy. | Mikkelsen et al. Clinical and Molecular Allergy 2010 8 2 http content 8 1 2 RESEARCH CMA CLINICAL AND MOLECULAR ALLERGY Open Access Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy Susan Mikkelsen1 Bo Martin Bibby2 Mette Konow Bogebjerg Dolberg1 Ronald Dahl1 Hans Jurgen Hoffmann1 3 Abstract Background Subcutaneous Immunotherapy SCIT modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity measured through the basophil activation test would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation using a Bland Altman plot and ROC curves. Methods Patients n 18 starting subcutaneous SCIT for wasp allergy with an up dosing scheme of 7 to 11 weeks were enrolled. Heparinised blood samples were drawn at weeks 1-4 7 and at the first maintenance visit. Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min and were stained with CD203c and CD63. Basophils were identified as CD203c leukocytes and the proportion of CD63 and CD203c cells were plotted against allergen concentration. A sigmoid curve was fitted to the points and the allergen concentration at which half of the maximal activation was achieved LC50 was calculated. In another series of experiments LC50 calculated in whole blood AP was subtracted from LC50 calculated with basophils suspended in plasma from a nonatopic donor HS to determine the protective effect of soluble factors in blood of patients treated with SCIT. Results Heparin blood basophil activation was similar through CD63 and CD203c. Basophils were significantly more sensitized three weeks after initiation of SCIT compared to baseline p 0 01 . The difference in LC50 increased by 1 04 LC50 units p 0 04 in patients that had just achieved maintenance dose compared with patients before .