tailieunhanh - Báo cáo y học: "Myocardial post-conditioning with DanshenGegen decoction protects against isoproterenolinduced myocardial injury via a PKCε/mKATPmediated pathway in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Myocardial post-conditioning with DanshenGegen decoction protects against isoproterenolinduced myocardial injury via a PKCε/mKATPmediated pathway in rats. | Wong et al. Chinese Medicine 2011 6 7 http content 6 1 7 CHINESE MEDICINE RESEARCH Open Access Myocardial post-conditioning with Danshen-Gegen decoction protects against isoproterenol-induced myocardial injury via a PKCe mKATP-mediated pathway in rats 1 1 1 2 7 7 2 1 1 Sze Man Wong Po Yee Chiu Hoi Yan Leung Limin Zhou Zhong Zuo Philip Y Lam Kam Ming Ko Abstract Background Danshen-Gegen decoction DG a Chinese herbal formula has been demonstrated to be effective for the treatment of coronary heart disease such as myocardial infarction. In the present study we investigated the effect of DG post-conditioning on isoproterenol ISO -induced myocardial injury in rats. Methods ISO was injected intraperitoneally 200 mg kg to induce acute 2-6 hours myocardial injury in adult female rats. DG 4 g kg was administered per oral immediately after the injection of ISO in the rats. Extent of myocardial injury was assessed by measurements of plasma enzyme activities. Myocardial mitochondrial glutathione antioxidant status lipid peroxidation and mitochondrial calcium ion loading and cytochrome c release were also measured. Effects of inhibitors of protein kinase C-epsilon PKCe ranslocation and mitochondrial ATP-sensitive potassium channel mKATP on myocardial post-conditioning by DG were investigated. Results ISO inflicted acute myocardial injury in the rats as evidenced by increased plasma enzyme activities. DG post-treatment alleviated the ISO-induced acute myocardial injury. Conclusion DG post-treatment protected the myocardium against ISO-induced acute injury in rats. The myocardial post-conditioning by DG is likely mediated by PKCs mKATP signaling pathway. Background Atherosclerosis which may occur in the coronary artery and is linked to the pathogenesis of coronary heart disease CHD involves the deposition of plaque-forming biomolecules cholesterol and triglycerides in particular onto the inner wall of arteries. The atherosclerotic coronary artery restricts .