tailieunhanh - Báo cáo khoa học: Transient RNA–protein interactions in RNA folding

The RNA folding trajectory features numerous off-pathway folding traps, which represent conformations that are often equally as stable as the native functional ones. Therefore, the conversion between these off-pathway struc-tures and the native correctly folded ones is the critical step in RNA fold-ing. | ẶFEBS Journal REVIEW ARTICLE Transient RNA-protein interactions in RNA folding Martina Doetsch Renee Schroeder and Boris Furtig Department of Biochemistry and Molecular CellBiology Max F. Perutz Laboratories University of Vienna Austria Keywords mode of binding proteins that promote RNA folding RNA chaperones RNA folding problem transient interactions Correspondence B. Furtig Department of Biochemistry and Molecular Cell Biology Max F. Perutz Laboratories University of Vienna Dr Bohrgasse 9 5 1030 Vienna Austria Fax 43 1 4277 9528 Tel 43 1 4277 52828 E-mail Re-use of this article is permitted in accordance with the Terms and Conditions set out at http onlineopen OnlineOpen_Terms Received 23 November 2010 revised 8 February 2011 accepted 11 March 2011 doi The RNA folding trajectory features numerous off-pathway folding traps which represent conformations that are often equally as stable as the native functional ones. Therefore the conversion between these off-pathway structures and the native correctly folded ones is the critical step in RNA folding. This process referred to as RNA refolding is slow and is represented by a transition state that has a characteristic high free energy. Because this kinetically limiting process occurs in vivo proteins called RNA chaperones have evolved that facilitate the re folding of RNA molecules. Here we present an overview of how proteins interact with RNA molecules in order to achieve properly folded states. In this respect the discrimination between static and transient interactions is crucial as different proteins have evolved a multitude of mechanisms for RNA remodeling. For RNA chaperones that act in a sequence-unspecific manner and without the use of external sources of energy such as ATP transient RNA-protein interactions represent the basis of the mode of action. By presenting stretches of positively charged amino acids that are positioned in