tailieunhanh - Báo cáo y học: " Research Upregulation of CD23 (FcεRII) Expression in Human Airway Smooth Muscle Cells (huASMC) in Response to IL-4, GM-CSF, and IL-4/GM-CSF"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Research Upregulation of CD23 (FcεRII) Expression in Human Airway Smooth Muscle Cells (huASMC) in Response to IL-4, GM-CSF, and IL-4/GM-CSF. | Clinical and Molecular Allergy BioMed Central Research Open Access Research Upregulation of CD23 FccRII Expression in Human Airway Smooth Muscle Cells huASMC in Response to IL-4 GM-cSf and IL-4 GM-CSF Joseph T Belleauh Radha K Gandhih Holly M McPherson and D Betty Lew Address Department of Pediatrics Children s Foundation Research Center at the Le Bonheur Children s Medical Center University of Tennessee Health Science Center 50 North Dunlap Street Rm401 WPT Memphis TN 38103 USA Email Joseph T Belleau - jcbelleau@ Radha K Gandhi - rgandhi@ Holly M McPherson - hMcPHERSoN@ D Betty Lew - dlew@ Corresponding author tEqual contributors Published 20 May 2005 Received 30 August 2004 Clinical and Molecular Allergy 2005 3 6 doi 86 1476-7961-3-6 Accepted 20 May 2005 This article is available from http content 3 1 6 2005 Belleau et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Airway smooth muscle cells play a key role in remodeling that contributes to airway hyperreactivity. Airway smooth muscle remodeling includes hypertrophy and hyperplasia. It has been previously shown that the expression of CD23 on ASMC in rabbits can be induced by the IgE component of the atopic serum. We examined if other components of atopic serum are capable of inducing CD23 expression independent of IgE. Methods Serum starved huASMC were stimulated with either IL-4 GM-CSF IL-13 IL-5 PGD2 LTD4 tryptase or a combination of IL-4 IL-5 IL-13 each with GM-CSF for a period of 24 h. CD23 expression was analyzed by flow cytometry western blot and indirect immunofluorescence. Results The CD23 protein expression was upregulated in huASMC in response to IL-4 .