tailieunhanh - Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery. | Kooren et al. Clinical Proteomics 2011 8 13 http content 8 1 13 CLINICAL PROTEOMICS RESEARCH Open Access Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery Joel A Kooren1 Nelson L Rhodus2 Chuanning Tang1 Pratik D Jagtap3 Bryan J Horrigan1 and Timothy J Griffin1 Correspondence tgriffin@umn. edu department of Biochemistry Molecular Biology and Biophysics University of Minnesota 321 Church St SE 6-155 Jackson Hall Minneapolis Minnesota 55455 USA Full list of author information is available at the end of the article 2 BioMed Central Abstract Introduction Early diagnosis of Oral Squamous Cell Carcinoma OSCC increases the survival rate of oral cancer. For early diagnosis molecular biomarkers contained in samples collected non-invasively and directly from at-risk oral premalignant lesions OPMLs would be ideal. Methods In this pilot study we evaluated the potential of a novel method using commercial PerioPaper absorbent strips for non-invasive collection of oral lesion exudate material coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery. Results Our evaluation focused on three core issues. First using an on-strip processing method we found that protein can be isolated from exudate samples in amounts compatible with large-scale mass spectrometry-based proteomic analysis. Second we found that the OPML exudate proteome was distinct from that of whole saliva while being similar to the OPML epithelial cell proteome demonstrating the fidelity of our exudate collection method. Third in a proof-of-principle study we identified numerous inflammation-associated proteins showing an expected increase in abundance in OPML exudates compared to healthy oral tissue exudates. These results demonstrate the feasibility of identifying differentially abundant proteins from exudate samples which is essential for biomarker .

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