tailieunhanh - Báo cáo y học: " A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions | Retrovirology BioMed Central Research Open Access A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection transgene delivery and generation of host genomic deletions Clifford L Wang 1 J Graeme Hodgson2 Tiffany Malek4 Finn Skou Pedersen3 and Matthias Wabl1 Address Department of Microbiology and Immunology University of California San Francisco CA USA 94143-0414 2Department of Laboratory Medicine University of California San Francisco CA USA 94143-0808 3Department of Molecular Biology and Department of Medical Microbiology and Immunology University of Aarhus Denmark DK-8000 and 4Fred Hutchinson Cancer Research Centre Department of Human Biology Seattle WA 98109 USA Email Clifford L Wang - cliffw@ J Graeme Hodgson - ghodgson@ Tiffany Malek - tmalek@ Finn Skou Pedersen - fsp@ Matthias Wabl - mutator@ Corresponding author Published 05 April 2004 Received 19 February 2004 Accepted 05 April 2004 Retrovirology 2004 1 5 This article is available from http content 1 1 5 2004 Wang et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background To generate a replication-competent retrovirus that could be conditionally inactivated we flanked the viral genes of the Akv murine leukemia virus with LoxP sites. This provirus can delete its envelope gene by LoxP Cre mediated recombination and thereby allow superinfection of Cre recombinase expressing cells. Results In our studies the virus repeatedly infected the cell and delivered multiple copies of the viral genome to the host genome the superinfected cells expressed a viral transgene on average twenty times more than non-superinfected cells. The insertion of multiple LoxP sites into the cellular genome also led to genomic deletions as .