tailieunhanh - Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists | Retrovirology BioMed Central Research Open Access Establishment of a novel CCR5 and CXCR4 expressing CD4 cell line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists Katrien Princen Sigrid Hatse Kurt Vermeire Erik De Clercq and Dominique Schols Address Rega Institute for Medical Research Katholieke Universiteit Leuven B-3000 Leuven Belgium Email Katrien Princen - Sigrid Hatse - Kurt Vermeire - Erik De Clercq - Dominique Schols - Corresponding author Published 08 March 2004 Received 23 February 2004 Accepted 08 March 2o04 Retrovirology 2004 1 2 This article is available from http content 1 1 2 2004 Princen et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background CCR5 and CXCR4 are the two main coreceptors essential for HIV entry. Therefore these chemokine receptors have become important targets in the search for anti-HIV agents. Here we describe the establishment of a novel CD4 cell line stably expressing both CCR5 and CXCR4 at the cell surface. Results In these cells intracellular calcium signalling through both receptors can be measured in a single experiment upon the sequential addition of CXCR4- and CCR5-directed chemokines. The cell line reliably supported HIV-I infection of diverse laboratory-adapted strains and primary isolates with varying coreceptor usage R5 X4 and R5 X4 and allows to investigate the antiviral efficacy of combined CCR5 and CXCR4 blockade. The antiviral effects recorded in these cells with the CCR5 antagonist SCH-C and the CXCR4 antagonist AMD3100 were similar to