tailieunhanh - Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis. | Zurawel et al. Clinical Proteomics 2011 8 1 http content 8 1 1 CLINICAL PROTEOMICS RESEARCH Open Access Proteomic profiling of the mesenteric lymph after hemorrhagic shock Differential gel electrophoresis and mass spectrometry analysis Achlox 71 miA ol1 Emocf E K ir tr tro PriL Pi Dolt 7 3 Unoan D IrM H f i3 tx3f i3r PcimIo3 K lr if iil T P 7Ìci i i lz M A cl 31 4 Ashley zurdwel EHiest E Ivioore Elik D reltz Jdllccll R Jordd Sdgdr Ddmle Monikd Dziecidtkowskd Anirban Bdnerjee3 dnd Kirk C Hdnsen1 2 4 Correspondence ernest. moore@ kirk. hdnsen@ Proteomics Fdcility University of Colorddo School of Medicine Aurord USA 3Depdrtment of Surgery Denver Hedlth Medicdl Center Denver USA Full list of duthor informdtion is dvdildble dt the end of the drticle 2 BioMed Central Abstract Experiments show thdt upon trdumdtic injury the composition of mesenteric lymph chdnges such thdt it initidtes dn immune response thdt cdn ultimdtely result in multiple orgdn dysfunction syndrome MODS . To identify cdndiddte protein medidtors of this process we cdrried out d qudntitdtive proteomic study on mesenteric lymph from d well characterized rdt shock model. We dndlyzed three dnimdls using dndlyticdl 2D differentidl gel electrophoresis. Intrd-dnimdl vdridtion for the mdjority of protein spots wds minor. Functiondl clustering of proteins revedled chdnges drising from several globdl cldsses thdt give novel insight into funddmentdl mechdnisms of MODS. Mdss spectrometry bdsed proteomic dndlysis of proteins in mesenteric lymph cdn effectively be used to identify cdndiddte medidtors dnd loss of protective dgents in shock models. Introduction Multiple organ dysfunction syndrome MODS remains a leading cause of death due to trauma. Traumatic injury leads to systemic influx that precipitates post-traumatic organ dysfunction liver lungs kidneys and heart 1 . Previous work has demonstrated that postshock mesenteric lymph PSML serves as the conduit by