tailieunhanh - Báo cáo y học: " Evidence for preferential copackaging of Moloney murine leukemia virus genomic RNAs transcribed in the same chromosomal site"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Evidence for preferential copackaging of Moloney murine leukemia virus genomic RNAs transcribed in the same chromosomal site | Retrovirology BioMed Central Research Open Access Evidence for preferential copackaging of Moloney murine leukemia virus genomic RNAs transcribed in the same chromosomal site Sergey A Kharytonchyk1 Alla I Kireyeva1 Anna B Osipovich1 2 and Igor K Fomin 1 Address laboratory of Cellular and Molecular Immunology Institute of Hematology and Blood Transfusion 223059 Minsk Republic of Belarus and 2Present address Department of Microbiology and Immunology Vanderbilt University School of Medicine Nashville TN37232 USA Email Sergey A Kharytonchyk - oklahomych@ Alla I Kireyeva - akireyeva@ Anna B Osipovich - Igor K Fomin - ikfomin@ Corresponding author Published 18 January 2005 Received 15 November 2004 Accepted 18 January 2005 Retrovirology 2005 2 3 doi 86 1742-4690-2-3 This article is available from http content 2 1 3 2005 Kharytonchyk et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Retroviruses have a diploid genome and recombine at high frequency. Recombinant proviruses can be generated when two genetically different RNA genomes are packaged into the same retroviral particle. It was shown in several studies that recombinant proviruses could be generated in each round of HIV-1 replication whereas the recombination rates of SNV and Mo-MuLV are 5 to 10-fold lower. The reason for these differences is not clear. One possibility is that these retroviruses may differ in their ability to copackage genomic RNAs produced at different chromosomal loci. Results To investigate whether there is a difference in the efficiency of heterodimer formation when two proviruses have the same or different chromosomal locations we introduced two .