tailieunhanh - Báo cáo y học: " Identification of the 15FRFG domain in HIV-1 Gag p6 essential for Vpr packaging into the virion"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of the 15FRFG domain in HIV-1 Gag p6 essential for Vpr packaging into the virion | Retrovirology BioMed Central Short report Open Access Identification of the l5FRFG domain in HIV-I Gag p6 essential for Vpr packaging into the virion Henghu Zhu 1 Heng Jian1 2 and Ling-Jun Zhao 1 Address institute for Molecular Virology St. Louis University School of Medicine St. Louis USA and 2Department of Plant Pathology China Agricultural University Beijing China Email Henghu Zhu - henghu_zhu@ Heng Jian - hengjian@ Ling-Jun Zhao - zhaol@ Corresponding author Published 13 September 2004 Received 09 September 2004 Accepted 13 September 2004 Retrovirology 2004 1 26 doi 1742-4690-1-26 This article is available from http content 1 1 26 2004 Zhu et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The auxiliary regulatory protein Vpr of HIV-1 is packaged in the virion through interaction with the Gag C-terminal p6 domain. Virion packaging of Vpr is critical for Vpr to exert functions in the HIV- 1 life cycle. Previous studies suggest that Vpr interacts with a Lxx 4 domain in p6 for virion packaging. In the present study mutational analysis of HIV-1 Gag p6 domain was performed in the context of the HIV-1 genome to examine the effect on virion packaging of Vpr. Surprisingly Ala substitutions for Leu44 and Phe45 in the Lxx 4 domain or deletion of the whole Lxx 4 domain amino acid 35-52 of the Gag p6 domain did not affect Vpr virion packaging. Vpr virion packaging was normal when amino acid 1-23 of the Gag p6 domain was preserved. Most importantly Ala substitutions for Phe15 Arg16 and Phe17 in the context of amino acid 1-23 of the Gag p6 domain abolished Vpr virion packaging. Single Ala substitutions for Phe15 and Phe17 also abolished Vpr virion packaging whereas .