tailieunhanh - Báo cáo y học: " Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae | Retrovirology BioMed Central Research Open Access Analysis of HIV-I Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae Xiao-Jian Yao1 2 Nicole Rougeau1 Ghislaine Duisit1 Julie Lemay1 and Éric A Cohen 1 Address 1Laboratoire de Rétrovirologie Humaine Département de Microbiologie et Immunologie Faculté de Médecine Université de Montreal Montreal Québec H3C 3J7 Canada and 2Current address Dept. of Medical Microbiology University of Manitoba Basic Medical Sciences Building 730 William Avenue Winnipeg Manitoba R3E 0W3 Canada Email Xiao-Jian Yao - yao2@ Nicole Rougeau - Duisit Ghislaine - Julie Lemay - Éric A Cohen - Corresponding author Published 16 August 2004 Received 30 June 2004 Accepted 16 August 2004 Retrovirology 2004 1 2I doi I 742-4690-1-21 This article is available from http content 1 1 21 2004 Yao et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The HIV-1 genome encodes a well-conserved accessory gene product Vpr that serves multiple functions in the retroviral life cycle including the enhancement of viral replication in nondividing macrophages the induction of G2 cell-cycle arrest and the modulation of HIV-l-induced apoptosis. We previously reported the genetic selection of a panel of di-tryptophan W -containing peptides capable of interacting with HIV-l Vpr and inhibiting its cytostatic activity in Saccharomyces cerevisiae Yao . J. Lemay N. Rougeau M. Clément S. Kurtz P. Belhumeur and E. A. Cohen J. Biol. Chem. v. 277 p. 488I6-48826 2002 . In this study we performed a mutagenic analysis of Vpr to identify sequence and or .