tailieunhanh - Molecular Biology Problem Solver 14

Molecular Biology Problem Solver 14. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | Streaking of Plates In general aerosol production is minimized by using smooth plates. Rough surfaces or bubbles in the agar can lead to excessive vibration of the loop. Spreading of samples with a sterile glass rod may minimize the production of aerosols on agar plates with rough surfaces. Pouring of Microbial Suspensions Following centrifugation pouring off the supernatant from a microbial suspension can result in aerosols. One safe way to minimize aerosol production is to use a funnel with the narrow end submerged into disinfectant in a large container. Use enough disinfectant to mediate the decontamination of the supernatant and a container large enough to avoid overflow. A volume of disinfectant equal to or greater than the amount of supernatant to be decontaminated should suffice. Rinse the funnel with more disinfectant to handle any residual supernatant clinging to the funnel wall. Pipetting Even for solutions deemed to be safe it is good practice to never mouth pipette. It is mandatory not to mouth-pipette a microbial solution even with a cotton-plugged pipette. Aspiration of organisms into the mouth can occur despite the cotton plug. Blowing out the last few droplets from a pipette can also form aerosols. Use either a manual or automatic pipetting aid to pipette. The discharge of the last few droplets using either manual or automatic pipette aids can result in aerosols so avoid this if possible. If it is necessary to discharge the entire contents of the pipette try to avoid spraying. Again for serious pathogens pipetting should be performed in a biosafety hood. Contaminated pipettes should be discarded into a container containing a sufficient volume of disinfectant to permit the complete immersion of the pipette. Are You Prepared to Deal with an Emergency Adequate preparation for an emergency requires both an appreciation for the potential hazards involved and knowledge of the resources available to handle the emergency. Such preparation should precede .