tailieunhanh - Drugs and Poisons in Humans - A Handbook of Practical Analysis (Part 16)

Introduction: Toluene, benzene, xylene and styrene are being widely used for chemical product materials, solvents and constituents of adhesives and paints, and thus sometimes cause poisoning incidents by inhaling their gas at chemical product-manufacturing factories. The abuse of thinner solvents, containing toluene, benzene and xylene, is now a social problem especially for young people. There are many crimes and poisoning incidents involving the thinner solvent abuse. Recently, styrene leaking from new building materials is an object of interest, because it causes the sick house syndrome. The above four solvents are rapidly absorbed into human body by inhalation, per os and. | Toluene benzene xylene and styrene by Xiao-Pen Lee and Keizo Sato Introduction Toluene benzene xylene and styrene are being widely used for chemical product materials solvents and constituents of adhesives and paints and thus sometimes cause poisoning incidents by inhaling their gas at chemical product-manufacturing factories. The abuse of thinner solvents containing toluene benzene and xylene is now a social problem especially for young people. There are many crimes and poisoning incidents involving the thinner solvent abuse. Recently styrene leaking from new building materials is an object of interest because it causes the sick house syndrome. The above four solvents are rapidly absorbed into human body by inhalation per os and percutaneously to cause various disturbances such as suppression of the central nervous system. In addition benzene and styrene are being regarded as carcinogenic compounds. For analysis of toluene benzene xylene and styrene the conventional GC methods with packed columns are being used 1-10 . In this chapter a method of their analysis for human blood using headspace wide-bore capillary GC is presented. Reagents and their preparation Standard solutions toluene benzene or each isomer a of xylene of special grade is dissolved in methanol to prepare each 1 mg mL solution. Internal standard IS solution aniline of special grade is dissolved in methanol to give 10 mg mL solution. GC conditions Column an Rtx-BAC2 wide-bore capillary column 30 m x mm i. d. film thickness pm Restek Bellefonte PA USA . GC conditions instrument a Shimazu GC-14B gas chromatograph Shimadzu Corp. Kyoto Japan detector FID column oven temperature 30 C 1 min 15 C min 240 C injection and detector temperature 240 C carrier gas He its flow rate mL min. Springer-Verlag Berlin Heidelberg 2005 150 Toluene benzene xylene and styrene Procedure i. A mL volume of whole blood 20 pL 200 pg of IS solution mL of distilled water and g of solid NaCl b are .