tailieunhanh - Introduction to molecular biology

Introduction to molecular biology ( in one hour!!) Stephen Edwards Overview. Overview of the cell Different sizes/functions Organised structure Bacterial genetics are different Eukaryotic cell DNA ontained in the nucleus Arranged in 22. | Introduction to molecular biology ( in one hour!!) Stephen Edwards Overview Overview of the cell Different sizes/functions Organised structure Bacterial genetics are different Eukaryotic cell DNA Contained in the nucleus Arranged in 22 chromosomes, plus two sex chromosomes Two copies of each identical to other humans, 98% to chimp! Around 2m DNA, enough to travel to sun and back 600 times! Therefore, very tightly packed 5' C-G-A-T-T-G-C-A-A-C-G-A-T-G-C 3' | | | | | | | | | | | | | | | 3' G-C-T-A-A-C-G-T-T-G-C-T-A-C-G 5' DNA function Carries the blueprint for life Duplication for new cells Make proteins for biological functions: Gene structure Genes must have: Exons Start site Control region mRNA DNA is grouped into threes (codons) AGTTTTGGGCCCAAA Start and stop codons mRNA is then modified and travels out of the nucleus mRNA splicing Alternative splicing Translation Uses mRNA as template to make proteins Occurs in ribosomes One codon corresponds to one amino acid Proteins . | Introduction to molecular biology ( in one hour!!) Stephen Edwards Overview Overview of the cell Different sizes/functions Organised structure Bacterial genetics are different Eukaryotic cell DNA Contained in the nucleus Arranged in 22 chromosomes, plus two sex chromosomes Two copies of each identical to other humans, 98% to chimp! Around 2m DNA, enough to travel to sun and back 600 times! Therefore, very tightly packed 5' C-G-A-T-T-G-C-A-A-C-G-A-T-G-C 3' | | | | | | | | | | | | | | | 3' G-C-T-A-A-C-G-T-T-G-C-T-A-C-G 5' DNA function Carries the blueprint for life Duplication for new cells Make proteins for biological functions: Gene structure Genes must have: Exons Start site Control region mRNA DNA is grouped into threes (codons) AGTTTTGGGCCCAAA Start and stop codons mRNA is then modified and travels out of the nucleus mRNA splicing Alternative splicing Translation Uses mRNA as template to make proteins Occurs in ribosomes One codon corresponds to one amino acid Proteins Huge proportion of cell (after water) Many functions: Structure (. collagen in bone) Enzymes Transmembrane receptors Hormones Four levels of structure Protein structure Protein interactions Proteins can form interations: Proteins (complexes, oligomers) mRNA DNA Proteins can bind to each other depending on their relative charges and structures Gene expression regulation Common terminology Genome/proteome Genotype/phenotype Pseudogene Novel protein/gene Putative gene Locus/Allele/Chromosome region Dominant/Recessive Homologous Symbol cDNA Motif Dalton (Da or kDa) Common abbreviations EST expressed sequence tag SNP single nucleotide polymorphism ORF open reading frame UTR untranscribed region Experimental techniques Cut, change, knockout genes Transgenics Sequence genes and proteins Over-express proteins Microarrays We have used the yeast two-hybrid system to identify proteins that interact with the intracellular portion of the hepatocyte growth factor (HGF) receptor (Met). We isolated a

• Sinh học
• thematic biology
• molecular biology
• genetic structure
• genetic
• biological material
• different sizes
• Scientific reports
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• Different sizes of feed stock
• Feed stock
• Performance evaluation of gasifier
• Briquette size considered
• Báo cáo lâm nghiệp hay
• cách trình bày báo cáo
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp
• Catalysts preparation
• FCC catalysts
• Structural properties
• Catalytic cracking
• Ammonia adsorbed
• Microporous Solids
• Glass size
• Tableware size
• Drinking behaviour
• Cross validation
• Micro drinking behaviours
• BMC Cancer
• Solid pulmonary nodule
• Lung cancer
• Computed tomography
• Solid lung cancerous nodules
• Pleural retraction
• Bronchial truncation
• Dynamic programing
• Supplemental irrigation
• On farm reservoir
• Soil moisture dynamics
• Yield response
• BMC Genomics
• X chromosome inactivation
• Extracellular matrix
• Sex differences
• Social interactions
• Prefrontal cortex
• BMC Veterinary Research
• Staphylococcus aureus
• Killing curves
• Minimum inhibitory concentration
• Antimicrobial treatment
• Radiation measurements
• Electron spin resonance
• Al hole centre
• Ti centres
• Peroxy centre
• Grain size
• Mesoporous materials
• Mixed matrix membranes
• Polymers of intrinsic microporosity
• Separation of carbon dioxide and methane
• Intrinsic microporosity