tailieunhanh - Đánh giá đa dạng di truyền loài Vên vên (Anisoptera costata Korth.) ở rừng nhiệt đới Đông Nam Bộ bằng chỉ thị phân tử SSR

Vên vên, Anisoptera costata, loài thực vật phân bố hẹp trong rừng nhiệt đới núi thấp Đông Nam Bộ, được đánh giá mức độ đa dạng di truyền trên cơ sở phân tích 9 locus microsatellite (SSR), từ 48 cá thể trưởng thành. Chín locus đều có kết quả đa hình, có 23 alen đã được ghi nhận cho tất cả locus nghiên cứu. Hàm lượng thông tin đa hình (PIC) cho mỗi cặp mồi đa hình trung bình 0,41 (0,24–0,51) và chỉ ra mức độ đa hình thấp. | TAP CHI SINH HOC 2019 41 2se1 amp 2se2 23 31 DOI 0866-7160 v41n2se amp DIVERSITY ASSESSMENT OF Anisoptera costata Korth. IN SOUTHEASTERN RAINFOREST USING SSR MARKERS Pham Mai Phuong1 Nguyen Vu Anh1 Nguyen Minh Tam2 Nguyen Thanh Tuan3 Bui Thi Tuyet Xuan4 Vu Dinh Duy1 1 Institute of Tropical Ecology Vietnam-Russia Tropical Centre Ha Noi Vietnam 2 Vietnam National Museum of Nature VAST Vietnam 3 Vietnam Forestry University - Dongnai Campus Dong Nai Vietnam 4 Institute of Ecology and Biological Resources VAST Vietnam Received 10 August 2019 accepted 28 September 2019 ABSTRACT The distribution of Anisoptera costata Korth. Dipterocarpaceae is restricted to lowland rainforests in southeastern Vietnam. For conservation of this species its genetic diversity was investigated based on nine microsatellites single sequence repeat SSR . In total forty-eight A. costata individuals in southeastern rainforest were analyzed. Polymorphism was found in all nine loci with a total of 23 alelles observed. The polymorphic information content PIC was in average indicating a low polymorphic value. Other measured values include discrimination power PD 0 43 resolving power Rp and Marker index MI were revealed. The SSR data of A. costata Korth. in southeast rainforest of Vietnam indicated a low genetic diversity NA Ho and He with positive inbreeding value FIS . This study also showed the importance of conserving the genetic resources of A. costata in southeastern rainforests. The threatened status of A. costata was related to a lack of genetic diversity. All existing populations remained in isolated small forest patches. An ex-situ conservation site shoukd be established with new big populations for this species from all genetic groups to improve the fitness of the species under different environmental stresses. Keywords Anisoptera costata genetic diversity microsatellites SSR species conservation. Citation Pham Mai Phuong .