tailieunhanh - Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design

Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not been well characterized. Here, we define rules that determine gRNA effectiveness for transcriptional repression in Saccharomyces cerevisiae. |

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