tailieunhanh - Urinary Exosomal miRNAs as biomarkers of bladder Cancer and experimental verification of mechanism of miR-93-5p in bladder Cancer

Bladder cancer (BC) is one of the most common malignancies globally. Early diagnosis of it can significantly improve patients’ survival and quality of life. Urinary exosomes (UEs)-derived miRNAs might be a promising biomarker for BC detection. | Lin et al. BMC Cancer 2021 21 1293 https s12885-021-08926-x RESEARCH Open Access Urinary Exosomal miRNAs as biomarkers of bladder Cancer and experimental verification of mechanism of miR-93-5p in bladder Cancer Hao Lin1 Xiaojun Shi1 Haoran Li1 Jialiang Hui1 Ruiyu Liu1 Zihao Chen1 Yuwen Lu2 and Wanlong Tan1 Abstract Background Bladder cancer BC is one of the most common malignancies globally. Early diagnosis of it can significantly improve patients survival and quality of life. Urinary exosomes UEs -derived miRNAs might be a promising biomarker for BC detection. Method A total of 12 patients with BC and 4 non-cancerous participants as healthy control were recruited from a single center between March 2018 and December 2019 as the discovery set. Midstream urine samples from each participants were collected and high-throughput sequencing and differentially expression analysis were conducted. Combined with miRNA expression profile of BC tissue from The Cancer Genome Atlas TCGA miRNAs biomarkers for BC were determined. Candidate miRNAs as biomarkers were selected followed by verification with a quantitative reverse-transcription polymerase chain reaction assay in an independent validation cohort consisting of 53 BC patients and 51 healthy controls. The receiver- operating characteristic ROC curve was established to evaluate the diagnostic performance of UE-derived miRNAs. The possible mechanism of miRNAs were revealed by bioinformatic analysis and explored in vitro experiments. Results We identified that miR-93-5p miR-516a-5p were simultaneously significantly increased both in UEs from BC compared with healthy control and BC tissue compared with normal tissue which were verified by RT-qPCR in the validation cohort. Subsequently the performance to discover BC of the miR-93-5p miR-516a- 5p was further verified with an area under ROC curve AUC of and respectively which was significantly higher than that of urine cytology AUC . Moreover .