tailieunhanh - Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica (Bioss.)

Biotechnological applications of fungal endophytes associated with medicinal plant AscIn this study, three different fungal endophytes were isolated from the medicinal plant of Asclepias sinaica and identified as Penicillium chrysogenum Pc_25, Alternaria alternata Aa_27 and the third fungal strain was described as sterile hyphae sinaica (Bioss.) | Annals of Agricultural Science 2015 60 1 95 104 H O S T E D BY Faculty of Agriculture Ain Shams University Annals of Agricultural Science locate aoas Biotechnological applications of fungal endophytes associated with medicinal plant Asclepias sinaica Bioss. Amr Hamza Fouda 1 Saad El-Din Hassan Ahmed Mohamed Eid 2 Emad El-Din Ewais 3 Botany and Microbiology Department Faculty of Science Al-Azhar University Cairo Egypt Received 19 March 2015 accepted 5 April 2015 Available online 12 May 2015 KEYWORDS Abstract Fungal endophytes associated with medicinal plants have potential role to promote plant Endophytes growth through different mechanisms. However the biological and ecological roles of fungal endo- Indole acetic acid phytes still totally unexplored. In this study three different fungal endophytes were isolated from Extracellular enzyme the medicinal plant of Asclepias sinaica and identified as Penicillium chrysogenum Pc_25 Medicinal plants Alternaria alternata Aa_27 and the third fungal strain was described as sterile hyphae Sh_26. It was recorded that these endophytes had various ability to produce several extracellular enzymes including amylase pectinase cellulase gelatinase xylanase and tyrosinase. Their antimicrobial activities against different specific test organisms were investigated as well. In addition both endo- phyte isolates . Sh_26 and Aa_27 were found to promote root growth higher than Pc_25 and con- trol treatments. These fungal isolates had a considerable impact on plant growth parameters including root elongation as a result of ammonia and IAA production. ª 2015 Production and hosting by Elsevier . on behalf of Faculty of Agriculture Ain Shams University. This is an open access article under the CC BY-NC-ND license http licenses by-nc-nd . Introduction plant tissues for all or part of their life cycle. Endophytes have the ability to colonize internal plant tissues of healthy leaves Endophytes are .