tailieunhanh - Comparison of DNA standards for real-time-pcr based quantification of lactobacillus acidophilus in dairy products

In this study, we evaluated the reliability of genomic DNAs and cloned recombinant plasmids as standard controls for absolute real-time PCR assay. The associated standard curves were constructed and used for the quantification of Lactobacillus acidophilus probiotics. | COMPARISON OF DNA STANDARDS FOR REAL-TIME PCR-BASED QUANTIFICATION OF LACTOBACILLUS ACIDOPHILUS IN DAIRY PRODUCTS Monir-Sadat Shakeri Address es Department of Food Biotechnology Research Institute of Food Science and Technology RIFST Mashhad Iran. Corresponding author https ARTICLE INFO ABSTRACT Received 21. 9. 2020 Probiotic bacteria are an essential part of the healthy gut microbiota. Fermented foods as potential sources of health-promoting bacteria Revised 26. 9. 2021 can regulate the intestinal microbial population. However the exact quantification of these bacteria in such multiple-strain matrixes Accepted 29. 9. 2021 continues to remain elusive. In this study we evaluated the reliability of genomic DNAs and cloned recombinant plasmids as standard Published controls for absolute real-time PCR assay. The associated standard curves were constructed and used for the quantification of Lactobacillus acidophilus probiotics. All stages from the design and construction of standards and related curves met the criteria for high-quality products. There were no significant differences between the two enumeration methods. However plasmid-based standard curves resulted Regular article in a lower detection limit than the curves of genomic DNA standards. Our findings showed that the non-linearized recombinant plasmids had long-term stability at high concentrations during storage at -20 C which strongly depended on the purification methods. We propose that the recombinant plasmid standards can supersede the traditional genomic DNA standards for accurate quantification of probiotic bacteria. Keywords Recombinant DNA Plasmid standard DNA calibrator Standard curve Bio-yoghurt INTRODUCTION properties Taylor et al. 2019 . Moreover it is undoubtedly associated with the choice and quality of standard controls. They should have excellent properties such Lactobacilli are common probiotics in food owning the specific .

• Sinh học
• Biotechnology and food sciences
• DNA standards
• Real time pcr
• Lactobacillus acidophilus in dairy products
• Recombinant DNA
• Plasmid standard
• Bacteriophages target
• Food borne pathogens in food industry
• Food borne illnesses
• Bacterial pathogens
• Design of novel pyrimidine analogs
• Synthesis of novel pyrimidine analogs
• Anti tubercular agents targeting thymidine kinase domain
• Molecular docking
• Thymidine kinase
• Nutritional factors influencing the biosynthesis
• Amyloid bioemulsifier beam1 applicable in food industry
• Plackett Burman
• Response surface method
• Solibacillus silvestris AM1 S silvestris
• Serotyping of salmonella isolates
• Salmonella isolates from food matrices
• Clinical specimens from Lebanon
• Multidrug resistant bacteria
• Foodborne diseases
• Maillard reaction marker
• Foods including jam
• Baby food
• Formation of chosen MRPs
• Nutritional content of souro
• Iranian traditional food
• In vitro evaluation
• Bile salt resistance
• Bacterial diversity in raw fish ceviche
• Raw fish ceviche
• Food safety
• The analyzed samples
• Chitosan nanoparticles
• Nanoparticles conjugate bacteriocin
• Lactococcus lactis subsp lactis
• Food borne pathogenic bacteria
• Magnetic micro dispersive solid phase extraction
• Food additive
• The FDA guidelines
• Self assembling manner
• Salt reduction
• Sensory preference
• Physicochemical parameters
• Image analysis
• External preference mapping
• Antitumor properties of vegetable oil extract
• Green microalga coelastrella sp
• Vegetable oil extract
• Antitumor activity
• Sunflower oil extract
• Taxonomic investigation of euplanktonic diatom communities
• New record
• High copper exposed area
• Harmful species Halamphora coffeiformis
• Virulence factors among
• ESBLs producing escherichia coli clinical isolates
• ESBLs producing escherichia coli
• Phylogenetic groups
• Pathogenicity islands PAIs
• Morphological of different cassava varieties
• Chemical characteristics of different cassava varieties
• The quality attributes
• Cassava varieties
• Quercetin functional parameters
• Dihydroquercetin on functional parameters
• Rat liver mitochondria
• Antioxidant activity
• Bioconversion of wine pomace by lentinus edodes
• Solid state system
• Lentinus edodes
• Phenolic compounds
• High polymer DNA
• Sspecies of hydrobionts
• Bovine spleen 4 11 times
• Degree of depolymerization
• Gray mold disease
• Visible/near infrared
• Early detection
• Multivariate data analysis
• Principal component analysis
• Usnea longissima
• Infectious disease
• Chromobacterium violaceum
• Evaluate antimicrobial
• Antiquorum sensing
• Locally available preservatives
• The fungus causing tomato fruit rot disease
• The shelf life of tomatoes
• Seasonal variations
• Fatty acid composition of common carp
• Open waters
• Fatty acid composition
• High PUFA content
• Burkholderia cepacia bam 12
• Mungbean field in Rajasthan augments plant growth
• Agricultural field soil of Gujarat
• Burkholderia cepacia catecholate
• Phosphate solubilizing bacteria
• Novel association between GnRHR gene
• Growth traits in awassi
• Single strand conformation polymorphism
• GnRHR gene exon 2
• Bio profiling
• Antimycobacterial bacteriocin
• Bacillus subtilis of ethnomedicinal origin
• Bioassay guided TLC techniques
• Bacteriocin producing lactic acid bacteria
• Salted dried camel meat
• Goat cheese against staphylococcus aureus
• Staphylococcus aureus