tailieunhanh - Screening and isolation of thermophilic fungi obtained from three selected compost wastes sites

This study was aimed to screen and isolate thermophilic fungi from three selected compost wastes sites (Palm oil mill, Wood chip piles and Abattoir dump), and identify potential producers of lipases, cellulases and proteases respectively. The thermophilic fungi were obtained from an equal depth of one meter in all the three collection sites with a temperature range of 40 to 45OC and later cultured at 70 OC. F | SCREENING AND ISOLATION OF THERMOPHILIC FUNGI OBTAINED FROM THREE SELECTED COMPOST WASTES SITES Omodele Ibraheem 1 Oluwafijimi Adetuyi 1 Kalejaiye Sheriff 1 Victoria John 1 Wuraola Fayela 1 John M. Ajayi 2 Address es 1 Plants for Biotechnological Resources Research Group Department of Biochemistry Federal University Oye-Ekiti PMB 373 Oye-Ekiti Ekiti State Nigeria. 2 Bioinformatics and Molecular Biology Unit Department of Biochemistry Federal University of Technology Akure Ondo State Nigeria. Corresponding author deleibraheem2007@ https ARTICLE INFO ABSTRACT Received 8. 4. 2020 Thermostable enzymes have found applications in the improvement of products quality in various industries. Despite the discovery of Revised 18. 4. 2021 several microorganisms as potential source of thermostable enzymes more biodiversity explorations are currently been carried out for Accepted 23. 4. 2021 high activity enzymes producing isolates. The discovery of new isolates having these characters will allow to efficiently carry out Published 1. 10. 2021 reactions in various industrial processes that involve extreme conditions. This study was aimed to screen and isolate thermophilic fungi from three selected compost wastes sites Palm oil mill Wood chip piles and Abattoir dump and identify potential producers of lipases cellulases and proteases respectively. The thermophilic fungi were obtained from an equal depth of one meter in all the three Regular article collection sites with a temperature range of 40 to 45 OC and later cultured at 70 OC. Further experimental screening analysis showed the presences of lipase cellulase and protease producing fungi present in samples collected from palm oil mill wood chips pills and abattoir dump site respectively. The isolated fungi were used for enzyme production in submerged fermentation for 10 days at 50 OC. Culture filtrate obtained from the medium of production were used for the assessment of enzymatic activity.

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