tailieunhanh - Extracellular glutaminase-free l-asparaginase from trichoderma viride F2: Purification, biochemical characterization and evaluation of its potential in mitigating acrylamide formation in starchy fried food
The purified L-asparaginase is monomeric with a molecular mass of 57 kDa and exhibited optimum activity at pH and 37 °C, which is relatively close to the human body's internal environment. The purified Lasparaginase showed high affinity and catalytic efficiency towards its natural substrate L-asparagine with Km and Vmax of mM and U/mL, respectively, and did not exhibit any intrinsic glutaminase activity |
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