tailieunhanh - Analyzing differentially expressed genes and pathways of Bex2-deficient mouse lung via RNA-Seq

According to gene enrichment analysis, PPAR pathway, cardiac muscle contraction, and cytokinecytokine receptor interaction were the most enriched pathways. Besides, the nuclear factor-κB signaling pathway and hematopoietic cell linage pathways were also enriched. Chronic obstructive pulmonary disease (COPD) is enriched among KEGG disease pathways. RT-qPCR assays confirmed the RNA-Seq results. This study opens a new window toward the biological functions of Bex2 in different systems. | Turkish Journal of Biology Turk J Biol 2021 45 588-598 http biology TÜBİTAK Research Article doi biy-2104-4 Analyzing differentially expressed genes and pathways of Bex2-deficient mouse lung via RNA-Seq 1 2 1 1 1 Noor BAHADAR Hanif ULLAH Salah ADLAT Rajiv KUMAR SAH May ZUN ZAW MYINT 1 1 1 1 3 Zin MAR OO Fatoumata BINTA BAH Farooq HAYEL NAGI Hsu HTOO Ahmad UD DIN 1 1 Xuechao FENG Yaowu ZHENG 1 Key Laboratory of Molecular Epigenetics Institute of Genetics and Cytology Northeast Normal University Changchun Jilin China 2 School of medicine Tsinghua University Beijing China 3 Drug Discovery Research Center Southwest Medical University Luzhou China Received Accepted Published Online Final Version Abstract Bex2 is well known for its role in the nervous system and is associated with neurological disorders but its role in the lung s physiology is still not reported. To elucidate the functional role of Bex2 in the lung we generated a Bex2 knock-out KO mouse model using the CRISPR-Cas9 technology and performed transcriptomic analysis. A total of 652 genes were identified as differentially expressed between Bex2- - and Bex2 mice out of which 500 were downregulated while 152 were upregulated genes. Among these DEGs Ucp1 Myh6 Coxa7a1 Myl3 Ryr2 RNaset2b Npy Enob1 Krt5 Myl2 Hba-a2 and Nrob2 are the most prominent genes. Myl2 was the most downregulated gene followed by Npy Hba-a2 Rnaset2b nr0b2 Klra8 and Ucp1. Tcte3 Eno1b Zfp990 and Pcdha9 were the most upregulated DEGs. According to gene enrichment analysis PPAR pathway cardiac muscle contraction and cytokine- cytokine receptor interaction were the most enriched pathways. Besides the nuclear factor-κB signaling pathway and hematopoietic cell linage pathways were also enriched. Chronic obstructive pulmonary disease COPD is enriched among KEGG disease pathways. RT-qPCR assays confirmed the RNA-Seq results. This study opens a new window toward the biological functions