tailieunhanh - Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm

The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introduction of wanted mutations. Unfortunately, it has been reported repeatedly that the sgRNA can also guide Cas9 to off-target sites where the DNA sequence is homologous to sgRNA. |

TỪ KHÓA LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
8    164    3    23-12-2024