tailieunhanh - Analytical parameters and validation of homopolymer detection in a pyrosequencing-based next generation sequencing system

Current technologies in next-generation sequencing are offering high throughput reads at low costs, but still suffer from various sequencing errors. Although pyro- and ion semiconductor sequencing both have the advantage of delivering long and high quality reads, problems might occur when sequencing homopolymercontaining regions, since the repeating identical bases are going to incorporate during the same synthesis cycle, which leads to uncertainty in base calling. |

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