tailieunhanh - Confirmation of hybridity using DNA-based markers is essential in chickpea (Cicer arietinum L.)

Confirmation of hybridity is essential to exclude selfed plants in plant breeding programmes. The morphological markers for confirmation of hybridity in chickpea are unavailable in closely related germplasm and these results in ambiguity in identification of true hybrids. In present study, cold tolerant parent ICC-16349 (donor) and cold susceptible parent GPF-2 (recipient) were crossed to generate 80 putative hybrids. | 2020 9 11 623-628 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 11 2020 Journal homepage http Original Research Article https Confirmation of Hybridity using DNA-based Markers is Essential in Chickpea Cicer arietinum L. Sanchit Thakur1 Jai Dev Sharma2 and Kamal Dev Sharma3 1 Department of Plant Breeding and Genetics 2Department of Tea Husbandry amp Technology 3 Department of Agricultural Biotechnology CSKHPKV Palampur India Corresponding author ABSTRACT Keywords Confirmation of hybridity is essential to exclude selfed plants in plant breeding Chickpea programmes. The morphological markers for confirmation of hybridity in chickpea are Hybridization SSR unavailable in closely related germplasm and these results in ambiguity in identification of True hybrids true hybrids. In present study cold tolerant parent ICC-16349 donor and cold susceptible parent GPF-2 recipient were crossed to generate 80 putative hybrids. The parents were Article Info screened using 51 simple sequence repeat SSR markers of which only one TA 180 exhibited polymorphism. Screening of 80 putative hybrids using TA 180 revealed that Accepted only 34 of the putative hybrids were true hybrids. The study indicated that in 07 October 2020 Available Online chickpea breeding programmes hybridity must be confirmed using DNA based markers to 10 November 2020 avoid inclusion of selfed plants as hybrids. Introduction Northern Syria where it is believed to be evolved from its progenitor Cicer reticulatum Chickpea Cicer arietinum L. 2n 2x 16 is Maesen 1987 . Chickpea is also known as one of the important pulse crop that was first Bengal gram Garbanzo bean Chana and grown in Turkey about 7000 BC Philologos Shonagalu in different chickpea producing 2005 . It is a self pollinated crop and belongs areas of the world. to kingdom Plantae order Fabales family Fabacae genus .

• Nông nghiệp
• True hybrids
• Confirmation of hybridity
• DNA based markers is essential
• Essential in chickpea
• Confirmation of hybridity in chickpea
• BMC Plant Biology
• Rosa hybrida L
• 2n pollen generation
• Strong competition
• Ploidy inheritable