tailieunhanh - Perform cluster analysis to assess the differences for storage seed protein profile in the cowpea mutants

The objective of the present investigation was therefore, to perform cluster analysis to assess the differences in the mutants of cowpea varieties RC-19 and RC-101 for this storage seed protein profile. | 2020 9 10 1302-1309 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 10 2020 Journal homepage http Original Research Article https Perform Cluster Analysis to Assess the Differences for Storage Seed Protein Profile in the Cowpea Mutants Madhu Choudhary K. Ram Krishna and Rajwanti Saran Department of Plant Breeding and Genetics . College of Agriculture SKN Agricultural University Jobner303329 Jaipur Rajasthan India Corresponding author ABSTRACT An investigation was carried out on 38 mutants derived from EMS mutagenesis of two cowpea varieties RC-19 and RC-101 to determine the variation in their profile of seed Keywords storage protein subunits through sodium dodecyl sulphate - polyacryl amide gel electrophoresis SDS-PAGE .The protein bands of the 40 genotypes parents and mutants Cowpea mutants could be placed in five distinct regions. The protein bands of region I II and V were Seed storage monomorphic and intensely stained and identified to have a MW between kD to 43 protein Clustering SDS-page kD. Only certain bands of region III and IV were polymorphic. The binary data generated from the polymorphic bands over the genotypes were used to compute Jaccard s similarity coefficients using NTSYS-pc software. The similarity matrix thus prepared was used to Article Info construct a dendrogram by UPGMA. The dendrogram distributed the 40 genotypes in 11 clusters. About 50 mutants were in one cluster and their parents in other clusters. One Accepted 12 September 2020 mutant in each group assumed unique place . mutant 30 dd of RC101 and mutant 37 Available Online kk of RC 19. Clustering seemed to be independent of the seed attributes studied. The 10 October 2020 protein content of the mutants was invariably reduced as compared to their parents and ranged from . It was concluded that results of the studies may be useful in