tailieunhanh - Nghiên cứu khả năng tái sinh và chuyển gen chỉ thị vào giống dưa chuột choka F1

Bài viết tiến hành với mục đích phát triển quy trình tái sinh và chuyển gen thông qua vi khuẩn A. tumefaciens ở cây dưa chuột tạo cơ sở cho nghiên cứu chuyển các gen mục tiêu mang các tính trạng quý cũng như chỉnh sửa hệ gen trên các giống dưa chuột tại Việt Nam. | TNU Journal of Science and Technology 226 01 83 - 91 STUDY IN VITRO REGENERATION AND TRANSFORMATION SYSTEMS OF CUCUMBER VARIETY CHOKA F1 USING GUS REPORTER GENE Hoang Thi Huyen Trang1 Hoang Khanh Linh4 Nguyen Thi Linh1 Trinh Dinh Duy1 Le Thi Nhu Thao1 2 3 Pham Bich Ngoc1 2 Chu Hoang Ha1 2 Do Tien Phat1 2 1Institute of Biotechnology VAST 2Graduate University of Sciences and Technology VAST 3Nam Bo Agriculture College 4VNU - Foreign Language Specialized School ARTICLE INFO ABSTRACT Received 09 01 2021 This study was performed to establish and develop efficient procedures for Agrobacterium-mediated transformation and regeneration of an inbred Revised 18 01 2021 cucumber cultivar which provides a potential protocol to transfer gene of Published 20 01 2021 interest into cucumber as well as further research on genome editing in cucumber in Vietnam. In this procedure cucumber seed sterilization was well KEYWORDS performed using 15 NaClO for 15 min. The frequency of multiple shoot induction was and the number of regenerated shoots per sample was Cucumber on MS basal medium added mg L BAP and 1 mg L ABA. The Regeneration rooting frequency ranged from 45 to 85 and the whole regenerated plants were formed on MS medium supplemented with 20 g L sucrose. The in vitro A. tumefaciens regenerated cucumber plants were successfully transferred and grown on Transformation vermiculite under greenhouse conditions with a survival rate of . Choka F1 These medium components and the regeneration procedure were successfully utilized to transfer gus reporter gene into the Choka F1 variety using the Agrobacterium-mediated method. The GUS staining and PCR analysis using specific primers demonstrated the presence and expression of gus gene in transgenic cucumber lines. The transformation frequency was preliminary recorded at . NGHIÊN CỨU KHẢ NĂNG TÁI SINH VÀ CHUYỂN GEN CHỈ THỊ VÀO GIỐNG DƯA CHUỘT CHOKA F1 Hoàng Thị Huyền Trang1 Hoàng Khánh Linh4 Nguyễn Thị Linh1 Trịnh