tailieunhanh - Phân lập và tuyển chọn các chủng Trichoderma có khả năng đối kháng vi nấm gây bệnh trên cây cam

Bài viết dựa trên đặc điểm hình thái và trình tự vùng ITS của rDNA, cả ba chủng , và được xác định thuộc loài Trichoderma asperellum. Ba chủng nấm Trichoderma tuyển chọn này có tiềm năng ứng dụng trong sản xuất chế phẩm sinh học dùng cho phòng trừ vi nấm gây bệnh trên cây cam và các cây trồng khác | VNU Journal of Science Natural Sciences and Technology Vol. 36 No. 3 2020 98-104 Original Article Isolation and Characterization of Trichoderma Strains Antagonistic Against Pathogenic Fungi on Orange Crops Vu Xuan Tao1 Tran Van Tuan2 1 Center of Experimental Biology National Center for Technological Progress Ministry of Science and Technology C6 Thanh Xuan Bac Thanh Xuan Hanoi Vietnam 2 Department of Microbiology Faculty of Biology VNU University of Science 334 Nguyen Trai Thanh Xuan Hanoi Vietnam Received 29 May 2020 Revised 24 June 2020 Accepted 29 June 2020 Abstract Agricultural production is greatly influenced by diseases caused by fungi. Penicillium digitatum is a common fungus that causes blue mold in citrus fruits. In addition Fusarium and Phytophthora species are also recognized as citrus pathogens involving in root rot and fruit rot. Currently the use of microbial bioproducts to control fungal pathogens is always prioritized for an organic and sustainable agriculture. Trichoderma species are considered as safe filamentous fungi that antagonize against many fungal plant pathogens. In this study 10 strains of Trichoderma were isolated and monitored for their antagonistic capacity towards the citrus pathogen P. digitatum. The strains Trichoderma and exhibited inhibitory efficacy of 95-100 against P. digitatum. Additionally these three strains also strongly suppressed the growth of two other common plant pathogens Fusarium oxysporum and Phytophthora capsici. Based on the morphological characteristics and the sequence analysis of the internal transcribed spacer ITS region of rDNA all three strains and were identified as Trichoderma asperellum. These Trichoderma strains represent promising potentials for applications in the production of bioproducts for the control of pathogenic fungi infecting citrus and other crops. Keywords Orange crops fungal antagonism Penicillium digitatum Fusarium oxysporum Phytophthora capsici Trichoderma .