tailieunhanh - Utilization of an unsterile medium for production of polyhydroxyalkanoate (PHA) by yangia SP. ND218

This study presents a new halophilic bacterial strain named Yangia sp. ND 218 which was isolated from the Giao Thuy mangrove, Nam Dinh Province, Vietnam which grew and accumulated PHA in a high salt medium. The possibility of designing an open (unsterile) fermentation process using Yangia sp. ND 218 was investigated. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2012 Vol. 57 No. 8 pp. 104-110 This paper is available online at http UTILIZATION OF AN UNSTERILE MEDIUM FOR PRODUCTION OF POLYHYDROXYALKANOATE PHA BY Yangia sp. ND218 Doan Van Thuoc Faculty of Biology Hanoi National University of Education Abstract. A moderate halophilic bacterium Yangia sp. ND218 which was isolated from mangrove soil samples in Giao Thuy District Nam Dinh Province was found to be able to synthesize co-polymers consisting mainly of 3-hydroxybutyrate and 3-hydroxyvalerate from different carbon sources. Batch fermentation for PHA production by Yangia sp. ND218 under unsterile condition with glucose as the carbon source was investigated. The maximum CDW of g L and PHA content of were obtained under unsterile conditions higher than those obtained under sterile conditions. The utilization of an unsterile medium for PHA production is a new discovery that could reduce the cost of polymer production. Keywords Yangia sp. ND218 polyhydroxyalkanoate unsterile condition. 1. Introduction Polyhydroxyalkanoates PHA are polyesters of hydroxyalkanoates accumulated intracellularly as carbon and energy storage materials in numerous microorganisms usually when grown under the limitation of a nutrient and in the presence of excess carbon 1 10 . PHA is typically produced as a polymer of 103 to 104 monomers which exists as discrete granules with about 5 to 13 granules per cell and with diameters of to µm. After being extracted from cells PHA possesses the common features of non-toxic biocompatible biodegradable and recyclable thermoplastics 8 . Poly 3-hydroxybutyrate PHB is the most common type of PHA synthesized by microorganisms and it is rigid and brittle 5 . However copolymers with varying monomer compositions can also be produced resulting in a high diversity of PHA molecules possessing a broad range of physico-chemical and mechanical properties. Received May 21 2012. .

• Giáo dục học
• Unsterile condition
• Unsterile medium
• Quantitative analysis
• Batch fermentation
• Polymer production
• Sterile conditions
• Different carbon sources
• Sheath blight
• Rhizoctonia solani
• Calcium pentothenate
• Nitrogen and vitamins
• Sources of carbon
• Sclerotia formation as well
• Carbon cycle
• pH of water
• Different sources in Kerala
• Water collected
• Seasonal effects on acidity
• Geographic seasonal effects
• Carbon sources
• Nitrogen sources
• Temperatures and pH
• Czapex’sdox agar
• Cyamopsidis (Rang and Rao)
• Banded leaf and sheath blight
• Pseudomonas fluorescens
• Bio control
• Wood biomass
• Characterization of biochar
• Sources of plant communities
• Delonix elata in pyrolysis unit
• Pyrogenic carbon
• Poly β hydroxybutyrate
• Rhizobium japonicum
• Different carbon
• Bradyrhizobium japonicum USDA 110
• Organic manures
• Organic carbon
• Bulk density
• Physico chemical properties
• Chilli cultivation