tailieunhanh - Isolation, selection and identification of Laccase-producing fungal strains and its dye decolorization ability

In this study, we used traditional methods to isolate and screen for fungal laccases that have potential for use in the textile industry (dye decolorization) and a molecular marker to identify studied samples. Fifteen strains of basidiomycetes were isolated and screened for laccase activities on phenol, guaiacol and RBBR. Five strains showed positive laccase activities on all three substrates. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2013 Vol. 58 No. 9 pp. 132-138 This paper is available online at http ISOLATION SELECTION AND IDENTIFICATION OF LACCASE-PRODUCING FUNGAL STRAINS AND ITS DYE DECOLORIZATION ABILITY Duong Minh Lam and Truong Thi Chien Faculty of Biology Hanoi National University of Education Abstract. Fungal studies have thus far received little attention from scientists in Vietnam especially regarding fungal diversity and their application in industrial processes. In this study we used traditional methods to isolate and screen for fungal laccases that have potential for use in the textile industry dye decolorization and a molecular marker to identify studied samples. Fifteen strains of basidiomycetes were isolated and screened for laccase activities on phenol guaiacol and RBBR. Five strains showed positive laccase activities on all three substrates. The strain CPB30 presented the largest halo zone diameter and was also fastest to decolorize RBBR in a culture broth within 64 hours of incubation . Using complete ITS sequence analysis the CPB30 strain was identified as being Trametes maxima T. maxima CPB30 . This strain expresses high laccase activity 614 -796 U mL which warrants further applied research. Keywords Laccase Trametes maxima RBBR decolorization Cuc Phuong. 1. Introduction Water pollution is a serious problem in many countries and this is especially true in developing countries where uncontrolled industrial processes are taking place. A type of pollutant that is currently being released into the environment is the dyes used in the fabric industry. Laccases benzenediol oxygen oxidoreductases EC have been efficiently used to decolor and detoxify dyes due to the oxidizing capacity of laccases on a wide variety of organic and inorganic compounds including diphenols polyphenols substituted phenols diamines and aromatic amines with a concomitant reduction of molecular oxygen to water 12 . Laccases