tailieunhanh - Cytotoxic constituents of the fungus kretzschmaria sandvicensis collected in Dien Bien, Vietnam

Three fungal metabolites, (1) O-methylsilvaticol, (2) orthosporin and (3) diplosporin were isolated from a rare fungus, Kretzschmaria sandvicensis, collected in Dien Bien, in northwest Vietnam. Their structures were established using a combination of high resolution MS (FT-ICR-MS) and 1H-NMR, 13C-NMR, HSQC and HMBC spectroscopy. They were evaluated for anticancer properties against the KB cell line. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2013 Vol. 58 No. 9 pp. 15-19 This paper is available online at http CYTOTOXIC CONSTITUENTS OF THE FUNGUS Kretzschmaria sandvicensis COLLECTED IN DIEN BIEN VIETNAM Ha Thi Nhung1 Bui Thi Thu Hien1 Luu Van Huyen2 and Dang Ngoc Quang1 1 Faculty of Chemistry Hanoi National University of Education 2 Hanoi University for Natural Resources and Environment Abstract. Three fungal metabolites 1 O-methylsilvaticol 2 orthosporin and 3 diplosporin were isolated from a rare fungus Kretzschmaria sandvicensis collected in Dien Bien in northwest Vietnam. Their structures were established using a combination of high resolution MS FT-ICR-MS and 1 H-NMR 13 C-NMR HSQC and HMBC spectroscopy. They were evaluated for anticancer properties against the KB cell line. It was found that compound 2 had moderate activity IC50 mL while compounds 1 and 3 had no cytotoxic activity IC50 gt 100µg mL . This is a first report on the chemical constituents of Kretzschmaria sandvicensis. Keywords Chemical constituents Kretzschmaria sandvicensis O-methylsilvaticol orthosporin diplosporin. 1. Introduction Fungi are among the largest groups of organisms of our globe. Out of an estimated million species it is thought that only 5 75 000 are now known 3 . The Xylariaceae family is one of the most important fungal groups especially with respect to the production of secondary metabolites. Of the Xylariaceae species it has been seen that their biological diversity correlates with the chemical diversity of their characteristic secondary metabolites 10 . This biodiversity is very high in the tropics 6 but of the 2200 fungal species that have been found in Vietnam 9 relatively few are Xylariaceae. If one discounts some recent work 4 5 it can be said that macrofingi including the Xylariaceae of Vietnam are still unknown. Kretzschmaria sandvicensis is a rare fungus of the Xylariaceae family that has never undergone chemical .